In vivo high-resolution synchrotron radiation imaging of collagen-induced arthritis in a rodent model

• Published in: Journal of synchrotron radiation Vol. 17; no. 3; pp. 393 - 399
• Main Authors: Choi, Chang-Hyuk, Kim, Hong-Tae, Choe, Jung-Yoon, Kim, Seong-Kyu, Choi, Gi-Whan, Jheon, SangHoon, Kim, Jong-Ki
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.05.2010
• Subjects: Animals; Arthritis; Arthritis, Experimental - diagnostic imaging; Biocompatibility; Biomedical materials; Collagen - adverse effects; collagen-induced arthritis (CIA) model; Disease Models, Animal; high-resolution phase-contrast radiography; Imaging; In vivo testing; Mice; Mice, Inbred DBA; Microstructure; microstructure of joint damage; Radiography; rheumatoid arthritis; Synchrotron radiation; synchrotron radiation imaging; Synchrotrons
• DOI: 10.1107/S0909049510009581
• ISSN: 1600-5775; 0909-0495

In vivo microstructures of the affected feet of collagen‐induced arthritic (CIA) mice were examined using a high‐resolution synchrotron radiation (SR) X‐ray refraction technique with a polychromatic beam issued from a bending magnet. The CIA models were obtained from six‐week‐old DBA/1J mice that were immunized with bovine type II collagen and grouped as grades 0–3 according to a clinical scoring for the severity of arthritis. An X‐ray shadow of a specimen was converted into a visual image on the surface of a CdWO4 scintillator that was magnified using a microscopic objective lens before being captured with a digital charge‐coupled‐device camera. Various changes in the joint microstructure, including cartilage destruction, periosteal born formation, articular bone thinning and erosion, marrow invasion by pannus progression, and widening joint space, were clearly identified at each level of arthritis severity with an equivalent pixel size of 2.7 µm. These high‐resolution features of destruction in the CIA models have not previously been available from any other conventional imaging modalities except histological light microscopy. However, thickening of the synovial membrane was not resolved in composite images by the SR refraction imaging method. In conclusion, in vivo SR X‐ray microscopic imaging may have potential as a diagnostic tool in small animals that does not require a histochemical preparation stage in examining microstructural changes in joints affected with arthritis. The findings from the SR images are comparable with standard histopathology findings.