Development and Validation of Targeted Gene Sequencing Panel Based Companion Diagnostic for Korean Patients with Solid Tumors

Recently, several panels using two representative targeting methods have been developed but they do not reflect racial specificity, especially for Asians. We have developed and analytically validated the Korean Pan-cancer Companion Diagnostic (CDX) Panel to apply targeted anticancer drugs to Korean...

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Bibliographic Details
Published inCancers Vol. 13; no. 20; p. 5112
Main Authors Min, Byung-Joo, Lee, Woo Seung, Seo, Myung-Eui, Lee, Kye-Hwa, Jeong, Seung-Yong, Ku, Ja-Lok, Kim, Yeul Hong, Shin, Sang-Won, Kim, Ju Han
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 12.10.2021
MDPI
Subjects
Antitumor agents
Cancer
Cancer therapies
Clinical trials
Copy number
custom panel
Design
Drugs
FDA approval
Genes
Genomes
Hospitals
Medical diagnosis
Mutation
Paraffin
Patients
pharmacogenomics
precision medicine
Solid tumors
targeted sequencing
Tumors
United States > US
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Summary:Recently, several panels using two representative targeting methods have been developed but they do not reflect racial specificity, especially for Asians. We have developed and analytically validated the Korean Pan-cancer Companion Diagnostic (CDX) Panel to apply targeted anticancer drugs to Korean patients based on the molecular characteristics of tumors using tumor samples without matched patient normal samples. The panel included 31 genes with reported single nucleotide variants, 9 genes with reported copy number variations, and 15 genes with predictive responses to targeted drugs under clinical testing, enabling the panel to be analyzed for the targets of 30 targeted anticancer drugs. It is cost-effective and optimized for cancer type-specific therapy in Korean cancer patients across solid cancer types while minimizing the limitations of existing approaches. In addition, the optimized filtering protocol for somatic variants from tumor-only samples enables researchers to use this panel without matched normal samples. To verify the panel, 241 frozen tumor tissues and 71 formalin-fixed paraffin-embedded (FFPE) samples from several institutes were registered. This gene screening method is expected to reduce test turnaround time and cost, making it a balanced approach to investigate solid cancer-related gene regions.
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The authors contributed equally to this work as first authors.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13205112