ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus

• Published in: Journal of microbiological methods Vol. 71; no. 2; pp. 114 - 122
• Main Authors: Krawczyk, Beata, Leibner, Justyna, Barańska-Rybak, Wioletta, Samet, Alfred, Nowicki, Roman, Kur, Józef
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.11.2007; Elsevier Science
• Subjects: Bacterial Typing Techniques - methods; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Child, Preschool; Cluster Analysis; DNA Fingerprinting - methods; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Family Health; Fundamental and applied biological sciences. Psychology; Furunculosis - epidemiology; Furunculosis - microbiology; Genetics; Genotype; Genotyping; Humans; Infant; Microbiology; Molecular Epidemiology; Nose - microbiology; PCR; Phenotyping; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Skin infection; Staphylococcal Skin Infections - epidemiology; Staphylococcal Skin Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - classification; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Transition Temperature; ADSRRS; Genotyping; SP PCR; Phenotyping; LM PCR; PCR MP; Skin infection; PCR; Staphylococcus aureus; Typing; Phylogenetic tree; Genotype; Polymerase chain reaction; Pulsed field electrophoresis; Fingerprint method; Bacteria; Micrococcales; Micrococcaceae; Skin; Intraspecific comparison
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2007.08.010

The present study was designed to evaluate the usefulness of two novel molecular typing methods, amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) and the PCR melting profile (PCR MP), for Staphylococcus aureus strain differentiation. Thirty-seven S. aureus strains isolated from patients with a history of furunculosis were studied. The strains were identified by determining several phenotypic properties and were genotyped using three differentiation methods: macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE), ADSRRS-fingerprinting, and PCR MP technique. In some cases the results obtained showed that the S. aureus isolated from the nose was identical to the one from the furuncle of the same patient. The same genotype was also identified for S. aureus strains isolated from two different members of a family with a history of recurrent furunculosis, although the active lesions were present in only one of them when the investigation was done. Results from strain genotyping illustrated that the recently developed ADSRRS-fingerprinting and PCR MP techniques are useful for studies of intraspecies genetic relatedness of S. aureus strains. They are as effective in discriminating closely related strains as the PFGE method, which is currently considered to be “the gold standard” for epidemiological studies.