Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family from Citrus sinensis in response to huanglongbing

• Published in: Frontiers in plant science Vol. 15; p. 1369883
• Main Authors: Huang, Guiyan, Hu, Yanan, Li, Fuxuan, Zuo, Xiru, Wang, Xinyou, Li, Fengyao, Li, Ruimin
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27.03.2024
• Subjects: Citrus sinensis; heavy metal-associated isoprenylated plant proteins; huanglongbing; phylogenetic analysis; Plant Science; susceptible gene; huanglongbing; phylogenetic analysis; Citrus sinensis; heavy metal-associated isoprenylated plant proteins; susceptible gene
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2024.1369883

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in maintaining heavy metal balance and responding to both biotic and abiotic stresses in vascular plants. However, the role of HIPPs in the response to Huanglongbing (HLB), a harmful disease of citrus caused by the phloem-colonizing bacterium Liberibacter asiaticus (CLas), has not been examined. In this study, a total of 26 genes were identified in , and they were grouped into 5 clades. The genes are distributed on 8 chromosomes and exhibited considerable synteny with found . Additionally, we analyzed the gene structure, conserved motifs and domains of the CsHIPPs. Various cis-acting elements related to plant hormones and stress responses were identified in the promoters of . Public transcriptome data and RT-qPCR analysis showed that the expression level of was significantly reduced in samples infected by CLas and ssp. . Furthermore, silencing the homologous gene of in increased the disease resistance of plants to bacteria. Our results provide a basis for functional studies of gene family in , highlighting their functions in bacterial resistance, and improve our understanding to the susceptibility mechanism of HLB.