The human androgen receptor is a 110 kDa protein

• Published in: Molecular and cellular endocrinology Vol. 63; no. 1; pp. 39 - 44
• Main Authors: van Laar, J.H., Vries, J.Bolt-de, Voorhorst-Ogink, M.M., Brinkmann, A.O.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.05.1989; Elsevier
• Subjects: Androgen; Biological and medical sciences; Cell Line; Cell Nucleus - analysis; Electrophoresis, Polyacrylamide Gel; Estrenes - metabolism; Fundamental and applied biological sciences. Psychology; Human; Humans; In situ photolabeling; LNCaP cells; Lymphoma - analysis; Lymphoma - ultrastructure; Male; man; Metribolone; Molecular Weight; prostate; Prostatic Neoplasms - analysis; Prostatic Neoplasms - ultrastructure; Receptor; Receptors, Androgen - analysis; Receptors, Androgen - metabolism; Steroid hormones. Cholecalciferol derivatives; Testosterone Congeners - metabolism; Tumor Cells, Cultured; Vertebrates: endocrinology; Human; LNCaP cells; Androgen; Receptor; Molecular weight; In situ photolabeling; Cell culture; Radiolabelling; Gel electrophoresis; Photoaffinity labelling; Male genital system; In vitro; Characterization; Proteins; Cell line; Prostate; Hormonal receptor
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/0303-7207(89)90079-8

The androgen receptor in human prostate carcinoma cells (LNCaP) has been studied after in situ photolabeling with [ 3H]R1881. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell extracts revealed the presence of two specifically labeled proteins of 110 kDa and 43 kDa. Both photolabeled proteins were stable in cell homogenates and generated different chymotryptic maps, suggesting that the two proteins were different. From ligand binding specificity studies could be concluded that the 110 kDa protein represents the androgen receptor. The 43 kDa protein showed binding specificity only for R1881. Both photolabeled proteins were recovered from LNCaP nuclei, but the 43 kDa protein showed a relatively higher affinity for nuclei than the 110 kDa protein. The function of this protein is unknown. It is concluded that the human androgen receptor is a protein with a molecular mass of 110 kDa.