Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer's disease

Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detec...

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Published inBio-protocol Vol. 10; no. 4; p. e3529
Main Authors Mondragón-Rodríguez, Siddhartha, Ordaz, Benito, Orta-Salazar, Erika, Díaz-Cintra, Sofia, Peña-Ortega, Fernando, Perry, George
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.02.2020
Bio-protocol LLC
Subjects
Biology
Methods
Innate behavior
Patch-clamp
Neurodegeneration
Pyramidal cells
Subthreshold oscillations and Alzheimer’s disease
Hippocampus
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Summary:Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of neurodegeneration. To this aim, we used a merge of patch-clamp with two hippocampal innate behavior tasks. With this experimental approach, whole-cell recordings of CA1 pyramidal cells, combined with hippocampal-dependent innate behaviors, have been crucial for evaluating the early mechanism of neurodegeneration and its consequences. Here, we present our protocol for whole-cell recordings of CA1 pyramidal cells and hippocampal dependent innate behaviors in an adolescent (p30) mice.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3529