Expression of MTLC gene in gastric carcinoma

• Published in: World journal of gastroenterology : WJG Vol. 9; no. 10; pp. 2160 - 2163
• Main Authors: Qiu, Guang-Bin, Gong, Li-Guo, Hao, Dong-Mei, Zhen, Zhi-Hong, Sun, Kai-Lai
• Format: Journal Article
• Language: English
• Published: United States Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning Province, China 01.10.2003; Baishideng Publishing Group Inc
• Subjects: Apoptosis - physiology; Cell Division - physiology; Cell Line, Tumor; Down-Regulation - physiology; Gastric Cancer; Gene Expression Regulation, Neoplastic; Humans; Nuclear Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms - pathology; Stomach Neoplasms - physiopathology; Transfection
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i10.2160

To investigate the expression of c-myc target from laryngeal cancer cells (MTLC) gene in gastric carcinoma (GC) tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823. RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues. BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418. Growth of cells expressing MTLC was observed daily by manual counting. Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay. The expression of MTLC mRNAs was down-regulated in 9(60%) of 15 cases of GC tissues. The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells. A marked acceleration of apoptosis was observed in MTLC-expressing cells. MTLC was down-regulated in the majority of GC tissues and could promote apoptosis of GC cell lines, which suggests that MTLC may play an important role in the carcinogenesis of gastric carcinoma.