Variation in the epigenetic silencing of FLC contributes to natural variation in Arabidopsis vernalization response

• Published in: Genes & development Vol. 20; no. 22; pp. 3079 - 3083
• Main Authors: Shindo, Chikako, Lister, Clare, Crevillen, Pedro, Nordborg, Magnus, Dean, Caroline
• Format: Journal Article
• Language: English
• Published: United States 15.11.2006
• Subjects: Alleles; Arabidopsis; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Chromatin - metabolism; Chromatin Immunoprecipitation; Cold Temperature; Epigenesis, Genetic; Flowers - physiology; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Genetic Variation; MADS Domain Proteins - genetics; MADS Domain Proteins - metabolism; Models, Genetic; Phenotype; Quantitative Trait Loci - genetics; Repressor Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 0890-9369; 1549-5477
• DOI: 10.1101/gad.405306

Vernalization, the cold-induced acceleration of flowering, involves the epigenetic silencing of the floral repressor gene FLOWERING LOCUS C (FLC). We investigated the molecular basis for variation in vernalization in Arabidopsis natural accessions adapted to different climates. A major variable was the degree to which different periods of cold caused stable FLC silencing. In accessions requiring long vernalization, FLC expression was reactivated following nonsaturating vernalization, but this reactivation was progressively attenuated with increasing cold exposure. This response was correlated with the rate of accumulation of FLC histone H3 Lys 27 trimethylation (H3K27me3). Thus, variation in epigenetic silencing of FLC appears to have contributed to Arabidopsis adaptation.