Gel-cast glass-ceramic tissue scaffolds of controlled architecture produced via stereolithography of moulds

• Published in: Biofabrication Vol. 4; no. 4; p. 045002
• Main Authors: Chopra, K, Mummery, P M, Derby, B, Gough, J E
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.12.2012
• Subjects: Actins - metabolism; Architecture; Cell Adhesion - physiology; Cell Growth Processes - physiology; Cell Nucleus - metabolism; Cells, Cultured; Ceramics - chemistry; Channels; Collagen Type I - metabolism; Electrochemical machining; glass ceramic; Glass ceramics; Humans; Microscopy; Molds; Moulds; Osteoblasts - cytology; scaffold; Scaffolds; Stereolithography; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 1758-5082; 1758-5090
• DOI: 10.1088/1758-5082/4/4/045002

Two glass-ceramic scaffolds with a simple cubic structure of 500 µm square ligaments and square channels of width 400 or 600 µm have been fabricated by gel-casting into moulds produced by stereolithography, followed by mould removal, polymer burnout and sintering. The scaffolds have crushing strengths of 41 ± 14 and 17 ± 5 Mpa, respectively. Using a method of assembling discrete slices of scaffold, we are able to study cell behaviour within a scaffold by disassembly. Both scaffold structures were seeded with primary human osteoblasts and these penetrate, adhere, spread and proliferate on the scaffold structure. The larger channel diameter scaffold shows a greater cell population (despite its smaller surface area) and more pronounced production of ECM components (collagen and mineralization) with increased time in culture. Studies of sectioned scaffolds show that cell density and ECM production decrease with depth and that the difference between the two scaffold architectures is maintained.