Adoptive transfer of natural killer cell activity in B6D2F1 mice challenged with Salmonella typhimurium

• Published in: Cellular immunology Vol. 135; no. 1; pp. 88 - 94
• Main Authors: Griggs, Nathan D., Smith, Randall A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.1991; Elsevier
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunotherapy, Adoptive; Killer Cells, Natural - immunology; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Organs and cells involved in the immune response; Rabbits; Salmonella Infections, Animal - immunology; Salmonella typhimurium; Salmonella typhimurium - immunology; Immune response; Rodentia; Immune regulation; Salmonella typhimurium; Adoptive transfer; Infection; Natural killer cell; Vertebrata; Immunosuppression; Mammalia; Depletion; Mouse; Bacteriosis; Bacteria; Salmonellosis; Enterobacteriaceae
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(91)90256-B

The purpose of this study was to extend our previous findings as to the role of murine NK cells in host protection to a challenge infection with virulent Salmonella typhimurium SR-11. B6D2F1 mice were depleted of NK cells with anti-asialo GM1 or a monoclonal antibody, anti-NK 1.1, followed by a salmonellae challenge. Significantly decreased numbers of splenic bacteria ( P < 0.005) in the NK cell-depleted mice were noted at 12, 24, and 48 hr postchallenging, compared to the sham-injected control animals. When Percoll gradient-enriched large granular lymphocytes (NK cells) were adoptively transferred to NK cell-depleted mice followed by challenging, the splenic bacterial numbers were comparable to those present in NK cell-intact, control mice. These data indicate that large granular lymphocytes (NK cells) are responsible for the down-regulation of the protective host response in mice challenged with the facultative intracellular parasite, S. typhimurium.