Effect of cationic ionophore monensin on the lipid composition and fluidity of rat epididymal spermatozoal membrane

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) Vol. 36; no. 1; pp. 79 - 87
• Main Authors: Sharma, Pinky, Kanwar, Shailender Singh, Sanyal, S. N.
• Format: Journal Article
• Language: English
• Published: United States Informa Healthcare 01.01.2013; Taylor & Francis
• Subjects: Animals; Calcium - metabolism; Cholesterol - analysis; dye binding; Epididymis - drug effects; fluorescence anisotropy; Gangliosides - analysis; lipid composition; Male; Membrane Fluidity - drug effects; Membrane Lipids - analysis; Monensin; Monensin - pharmacology; rat epididymis; Rats; Rats, Wistar; Sodium Ionophores - pharmacology; Spermatozoa - drug effects; spermatozoal membrane
• ISSN: 0148-0545; 1525-6014
• DOI: 10.3109/01480545.2011.650174

The present study was aimed at exploring the effect of monensin, an antibiotic carboxylic polyether ionophore specific for Na+, on the structural, chemical, and physiological changes of the epididymal sperm of Wistar rats. Animals received monensin at the dose of 3.5 mg/kg body weight daily orally for 70 days, a treatment duration that corresponds to the spermatogenic cycle in rats. At the end of the treatment regime, three regions of the epididymis were separated and the spermatozoa were collected. The plasma membranes of the spermatozoa were isolated and lipid composition, such total lipid, phospholipid, cholesterol, and ganglioside-sialic acid, was studied. Membrane dynamic behavior was investigated by lipid translational fluidity by pyrene excimer formation and rotational diffusion by diphenyl hexatriene polarization and anisotropy parameter. Structural changes in membrane were also evaluated by the dye-binding study with anilino naphthalene sulphonic acid. The results showed marked changes in lipid compositions, fluidity parameters, and kinetics of fluorescent dye binding in the epididymis, and it can be concluded that monensin, by interfering with normal physiological changes in spermatozoal maturation, may provide the basis of certain molecular intervention in the fertilizing capability of the epididymal spermatozoa and thereby may induce antifertility properties in male rats.