Decapitation-induced changes in inositol phosphates in rat brain

• Published in: Biochemical and biophysical research communications Vol. 167; no. 3; pp. 1294 - 1301
• Main Authors: Lin, Teng-Nan, Sun, Grace Y., Premkumar, Noel, MacQuarrie, Ronald A., Carter, Steven R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.03.1990; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Brain - metabolism; Brain Ischemia - metabolism; Cerebral Cortex - metabolism; Decerebrate State; Fundamental and applied biological sciences. Psychology; Inositol - metabolism; Inositol Phosphates - isolation & purification; Inositol Phosphates - metabolism; Kinetics; Lipids; Miscellaneous; Other biological molecules; Postmortem Changes; Rats; Rats, Inbred Strains; Time Factors; InsP; InsP2; PtdIns(4,5)P2; InsP3; PtdIns; PtdInsP; Brain; Radiolabelling; Rat; Rodentia; HPLC chromatography; Decapitation; Lipids; Inositol phosphate; Ion exchange chromatography; Vertebrata; Mammalia; Enzymatic digestion; Kinetics; Quantitative analysis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(90)90664-9

Decapitation resulted in a time-dependent production of inositol phosphates in rat brain. This production was analyzed by measuring both the radioactivity and the concentrations of inositol phosphates generated from [3H]inositol-labeled phospholipids. Both measurements produced the same time-dependent changes, including a rapid decrease in inositol 1,4,5-trisphosphate within 1.5 min, a 6-fold increase in inositol 1,4-bisphosphate to a maximum at 1.5 min, a 5-fold rise in inositol 4-monophosphate to a maximum at 2.5 min, and little change in inositol 1-monophosphate. The temporal changes in the mass and radioactivity of these compounds, together with the decrease in labeling of phosphatidylinositol 4,5-bisphosphates, support the idea that the inositol phosphates originate from the hydrolysis of phosphatidylinositol 4,5-bisphosphates and not from either the direct hydrolysis of phosphatidylinositol 4-phosphates or phosphatidylinositols.