The role of carbohydrate in erythropoietin action

The carbohydrate composition of human erythropoietin (epo) was determined by micro-GLC. Enzymic removal of most of the sugars results in aggregation of glycosidase-treated epo, loss of biological activity when assayed in mice, and retention of activity when assayed in marrow cell cultures or by RIA....

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Bibliographic Details
Published inEndocrinology (Philadelphia) Vol. 116; no. 6; p. 2293
Main Authors Dordal, M S, Wang, F F, Goldwasser, E
Format Journal Article
LanguageEnglish
Published United States 01.06.1985
Subjects
Carbohydrates - analysis
Carbohydrates - physiology
Erythropoietin - analysis
Erythropoietin - pharmacology
Glycoside Hydrolases - pharmacology
Molecular Weight
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Summary:The carbohydrate composition of human erythropoietin (epo) was determined by micro-GLC. Enzymic removal of most of the sugars results in aggregation of glycosidase-treated epo, loss of biological activity when assayed in mice, and retention of activity when assayed in marrow cell cultures or by RIA. Endoglycosidase F causes the removal of most of the carbohydrates indicating that the oligosaccharides are asparagine linked. The lack of O-linked sugar is confirmed by the absence of N-acetylgalactosamine. These findings indicate that the oligosaccharide portion of epo, although required for action in vivo, is not required for interaction with the target cells of the blood-forming system.
ISSN:0013-7227
DOI:10.1210/endo-116-6-2293