A combined bovine herpesvirus 1 gB-gD DNA vaccine induces immune response in mice

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 28; no. 2; pp. 155 - 166
• Main Authors: Caselli, E., Boni, M., Di Luca, D., Salvatori, D., Vita, A., Cassai, E.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.03.2005; Elsevier
• Subjects: Administration, Intranasal; Animals; Antibodies, Viral - blood; Antigènes secrètes; BHV-1; Biological and medical sciences; Bovine herpesvirus 1; Cattle; Cattle Diseases - immunology; Cattle Diseases - prevention & control; Cattle Diseases - virology; DNA immunization; DNA, Viral - chemistry; DNA, Viral - genetics; Enzyme-Linked Immunosorbent Assay; Female; Fundamental and applied biological sciences. Psychology; Glycoprotein genes; Gènes de glycoprotéines; Herpesviridae Infections - immunology; Herpesviridae Infections - prevention & control; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus; Herpesvirus 1, Bovine - genetics; Herpesvirus 1, Bovine - immunology; Herpesvirus Vaccines - administration & dosage; Herpesvirus Vaccines - genetics; Herpesvirus Vaccines - immunology; Immunization; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Neutralization Tests; Plasmids; Polymerase Chain Reaction; Secreted antigens; Vaccines, Combined - administration & dosage; Vaccines, Combined - genetics; Vaccines, Combined - immunology; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Vaccins d'ADN; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Proteins - genetics; Viral Proteins - immunology; Virology; BHV-1; Antigènes secrètes; Vaccins d'ADN; Glycoprotein genes; DNA immunization; Secreted antigens; Gènes de glycoprotéines; Immune response; Microbiology; Herpesviridae; Alphaherpesvirinae; Rodentia; Glycoprotein; Bovine herpesvirus 1; Genetic vaccine; Virus; Antigen; Infection; Vertebrata; Mammalia; Immunology; Gene; Mouse; Veterinary
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2004.10.001

Although DNA vaccines have several advantages over conventional vaccines, antibody production and protection are often not adequate, particularly in single plasmid vaccine formulations. Here we assessed the potential for a combined vaccine based on plasmids encoding the membrane-anchored or secreted forms of bovine herpesvirus type 1 (BHV-1) glycoprotein B and D (gB and gD) to induce neutralizing and cell mediated immune responses in mice. Animals were injected by intramuscular, subcutaneous and intranasal routes. Mice immunized with the combined vaccine containing the secreted forms of BHV-1 glycoproteins developed higher titers of anti-BHV-1 neutralizing antibodies, compared to wild type gB/gD combined plasmids and to single plasmid injected groups. Cellular immunity was also developed in mice immunized with combined vaccines, whereas low or no response were observed in single plasmid injected animals. The data suggest the potential use of this combined vaccine in in vivo trials of calves, in order to evaluate its protective efficacy. Les vaccins d'ADN présent plusieurs avantages comparés aux vaccins conventionnels, mais la production et la protection des anticorps ne sont pas souvent suffisantes, en particulier en ce qui concerne les vaccins obtenus par des plasmides individuels. Nous avons évalué la capacité d'induire chez la souris une réponse immunitaire de neutralisation et cellulaire d'un vaccin combiné. Il combine des plasmides codant pour les glycoprotéines B et D (gB et gD), liées à la membrane ou sécrétées, de l'herpesvirus bovin de type 1 (BHV-1). Les animaux ont été immunisés par les voies intramusculaire, sub-cutanée et intranasale. Les souris immunisées avec le vaccin combiné contenant les formes sécrétées des glycoprotéines du BHV-1 ont développé des titres plus élevés des anticorps anti-BHV-1 neutralisants, comparés avec les groups immunisés par les vaccins contenant soit les gènes sauvages de type gB et gD soit les plasmides individuels. Les souris immunisés par les vaccins combinés ont développé aussi une immunité cellulaire, tandis que une réponse assez bas ou absente a été observée chez les animaux immunisés par les plasmides individuels. Les donnés suggèrent que les vaccins combinés peuvent être utilisés dans des expérimentations in vivo chez les bovins pour évaluer leur efficacité protectrice.