Molecular karyotype single nucleotide polymorphism analysis of early fetal demise

• Published in: Systems biology in reproductive medicine Vol. 59; no. 4; pp. 227 - 231
• Main Authors: Li, Gang, Liu, Yan, He, Nan-nan, Hu, Lin-li, Zhang, Yi-le, Wang, Yang, Dong, Fang-li, Guo, Yi-hong, Su, Ying-chun, Sun, Ying-pu
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01.08.2013; Taylor & Francis
• Subjects: Abortion, Spontaneous - diagnosis; Abortion, Spontaneous - genetics; Adult; chromosomes; Embryo Transfer; Female; Fertilization in Vitro; Humans; Karyotyping - methods; microarray; Polymorphism, Single Nucleotide; Pregnancy; Preimplantation Diagnosis - methods; preimplantation genetic diagnosis; single nucleotide polymorphism; Sperm Injections, Intracytoplasmic; Tissue Array Analysis - methods
• ISSN: 1939-6368; 1939-6376; 1939-6376
• DOI: 10.3109/19396368.2012.750696

We explored the application of single nucleotide polymorphism microarray (SNP array) in molecular karyotype analysis for early spontaneous abortion detection in assisted reproductive technology (ART). SNP array was performed in 81 cases. Of the 81 cases, 16 experienced natural conception (NC) and 65 were pregnant by ART. Of the 65 cases, 4 underwent artificial insemination (AI), 32 fresh in vitro fertilization-embryo transfer (IVF-ET), 9 fresh intracytoplasmic sperm injection (ICSI), and 20 thawed embryo transfer. In the 81 cases examined 69.1% displayed an abnormal molecular karyotype. In the subjects greater than 35 years of age, the abnormal molecular karyotype rate was 87.5% higher compared to 61.4% in younger individuals (P < 0.05). There was no significant difference in the abnormal molecular karyotype rate or type between ART (64.6%) and NC (87.5%). Compared with traditional cytogenetic diagnosis, the SNP array can identify a greater number of abnormal karyotypes.