Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

• Published in: Soil systems Vol. 5; no. 2; p. 26
• Main Authors: Djebaili, Rihab, Pellegrini, Marika, Rossi, Massimiliano, Forni, Cinzia, Smati, Maria, Del Gallo, Maddalena, Kitouni, Mahmoud
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 10.04.2021
• Subjects: 1-Aminocyclopropane-1-carboxylate deaminase; Abiotic stress; Acetic acid; Acids; Actinobacteria; Actinomycetes; Ammonia; Chlorophyll; durum wheat; Experiments; greenhouse experimentation; Greenhouses; growth and development; halotolerance; Hydrocyanic acid; hydrogen cyanide; Hypotheses; indole acetic acid; Indoles; Inoculation; Parameters; PGPB; phosphates; Plant growth; Proline; Proteins; Salinity; Salinity tolerance; Salt; salt stress; salt tolerance; Salts; Seeds; Sodium chloride; soil; Solubilization; stress tolerance; Triticum durum; Triticum turgidum subsp. durum; Wheat; United States > US
• ISSN: 2571-8789; 2571-8789
• DOI: 10.3390/soilsystems5020026

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.