Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

• Published in: Journal of microbiological methods Vol. 78; no. 1; pp. 104 - 106
• Main Authors: Sandberg, Malena E., Schellmann, Denise, Brunhofer, Gerda, Erker, Thomas, Busygin, Igor, Leino, Reko, Vuorela, Pia M., Fallarero, Adyary
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2009; Elsevier
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biofilms; Biological and medical sciences; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Fundamental and applied biological sciences. Psychology; Microbial Viability; Microbiology; Oxazines - chemistry; Oxazines - metabolism; Resazurin; Screening; Staining and Labeling - methods; Staphylococcus aureus; Staphylococcus aureus - chemistry; Staphylococcus aureus - physiology; Xanthenes - chemistry; Xanthenes - metabolism; Resazurin; Screening; Biofilms; Staphylococcus aureus; Biofilm; Staining; Bacteria; Micrococcales; Micrococcaceae; Method; Detection; Quantitative analysis
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2009.04.014

Staining of Staphylococcus aureus biofilms with 20 µM resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated.