The antioxidant activity of polysaccharides from Armillaria gallica

The purpose of this study was to investigate the antioxidant activity of polysaccharides. It explored whether polysaccharides (AgP) could prevent HepG cells from H O -induced oxidative damage. The results demonstrated that HepG cells were significantly protected by AgP, and efficiently suppressed th...

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Published inFrontiers in nutrition (Lausanne) Vol. 11; p. 1277877
Main Authors Su, Peiwen, Qiu, Honghao, Liang, Lishan, Weng, Luo, Liu, Yingjie, Liu, Jiajun, Wu, Liyan, Meng, Fanxin
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 14.02.2024
Subjects
antioxidant
antioxidant capacity
Armillaria gallica
HepG2
Nutrition
polysaccharides
polysaccharides
HepG2
antioxidant
Armillaria gallica
antioxidant capacity
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Summary:The purpose of this study was to investigate the antioxidant activity of polysaccharides. It explored whether polysaccharides (AgP) could prevent HepG cells from H O -induced oxidative damage. The results demonstrated that HepG cells were significantly protected by AgP, and efficiently suppressed the production of reactive oxygen species (ROS) in HepG cells. Additionally, AgP significantly decreased the abnormal leakage of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) caused by H O , protecting cell membrane integrity. It was discovered that AgP was also found to regulate the activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX), while reducing malondialdehyde (MDA), thus protecting cells from oxidative damage. According to the flow cytometry analysis and measurement of caspase-3, caspase-8, and caspase-9 activities, AgP could modulate apoptosis-related proteins and attenuate ROS-mediated cell apoptosis.
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Edited by: José Pinela, Instituto Politécnico de Bragança, Portugal
Reviewed by: Amélia M. Silva, University of Trás-os-Montes and Alto Douro, Portugal
Junxiang Zhu, Qingdao Agricultural University, China
ISSN:2296-861X
2296-861X
DOI:10.3389/fnut.2024.1277877