Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277

To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/μg of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experi...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 66; no. 1; pp. 174 - 176
Main Authors Ravindran, C.R. Marutha, Suguna, S., Shanmugasundaram, S.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier B.V 01.07.2006
Elsevier Science
Subjects
Bacteriology
Biofertilizer
Biological and medical sciences
Cyanobacteria
Cyanobacteria - genetics
Electroporation
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
Genetics
Kanamycin
Microbiology
Oscillatoria
Periplasmic nuclease
Plasmids - genetics
Transformation, Bacterial
Biofertilizer
Electroporation
Kanamycin
Oscillatoria
Cyanobacteria
Periplasmic nuclease
Plasmid
Resistance
Transfer
Bacteria
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Summary:To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/μg of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2005.11.011