Genetic characterization of the H9N2 influenza viruses circulated in the poultry population in Israel

• Published in: Comparative Immunology, Microbiology and Infectious Diseases Vol. 29; no. 4; pp. 207 - 223
• Main Authors: Perk, Shimon, Banet-Noach, Caroline, Shihmanter, Ester, Pokamunski, Shimon, Pirak, Michael, Lipkind, Michael, Panshin, Alexander
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.07.2006; Elsevier
• Subjects: Amino Acid Motifs; Amino Acid Sequence; Analyse phylogénique; Animals; Avian influenza virus; Base Sequence; Biological and medical sciences; Chickens; Cleavage site; Disease Outbreaks - veterinary; Fundamental and applied biological sciences. Psychology; Gène de l'hémaglutinine; H9N2 subtype; Hemagglutination Tests; Hemagglutinin gene; Hemagglutinins, Viral - chemistry; Hemagglutinins, Viral - genetics; Influenza A Virus, H9N2 Subtype - genetics; Influenza in Birds - epidemiology; Influenza in Birds - virology; Influenza virus; Israel - epidemiology; Microbiology; Miscellaneous; Molecular Sequence Data; Neuraminidase - antagonists & inhibitors; Phylogenetic analysis; Phylogeny; Poultry Diseases - epidemiology; Poultry Diseases - virology; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Viral - chemistry; RNA, Viral - genetics; Sequence Alignment; Site de clivage; Turkeys; Virology; Virus de la grippe aviaire de type H9N2; Asia; Israel; Phylogenetic analysis; Gène de l'hémaglutinine; Cleavage site; Hemagglutinin gene; Avian influenza virus; Analyse phylogénique; H9N2 subtype; Site de clivage; Virus de la grippe aviaire de type H9N2; Farming animal; Microbiology; Hemagglutinin; Poultry; Orthomyxoviridae; Phylogeny; Infection; Virus; Influenzavirus A; Immunology; Gene; Avian influenzavirus; Viral disease; Influenza; Genetics; Veterinary; Subtype
• ISSN: 0147-9571; 1878-1667; 1365-2567
• DOI: 10.1016/j.cimid.2006.06.004

The partial nucleotide sequences of the hemagglutinin (HA) genes of 72 H9N2 influenza viruses isolated from chickens and turkeys in Israel during the period 2000–2005 were genetically analyzed. The isolates possessed the three types of amino acid motif –R–S–S–R/G–L–, –R–S–N–R/G–L–, and –R–S–K–R/G–L– at the cleavage site of HA. Phylogenetic analyses showed that all Israeli isolates belonged to the same group which further divided into three closely related sub-groups. The HA genes of these isolates were related to the HA gene of A/chicken/Germany/R45/98 isolated from chicken in Germany in 1998. La séquence nucléotidique partielle du gène de l’hémaglutinine de 72 virus influenza de type H9N2; isolés de poulets et de dindes durant les années 2000–2005; a été analysée. Les isolats possèdent trois types de motifs d’acide aminées: -R-S-S-R/G-L-, -R-S-N-R/G-L-, et –R-S-K-R/G-L- au niveau du site de clivage de l’hémaglutinine. L’analyse phylogénique montre que tous les isolats israéliens appartiennent au même groupe, celui-ci se sub-divise ultérieurement en trois sous-groupes étroitement apparentés. Les gènes de l’hémaglutinine de ces isolats sont apparentés à celui du virus A/chicken/Germany/R45/98 isolé d’un poulet en Allemagne en 1998.