Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation

• Published in: Pharmaceutical biology Vol. 53; no. 3; pp. 437 - 445
• Main Authors: Begum, Rayhana, Sharma, Manju, Pillai, K. K., Aeri, Vidhu, Sheliya, Manjur Ali
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare USA, Inc 01.03.2015; Informa Healthcare
• Subjects: Animals; Anti-inflammatory; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Biomarkers - blood; Carrageenan - toxicity; Edema - blood; Edema - chemically induced; Edema - drug therapy; IL-1β; Inflammation - blood; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - blood; Lecythidaceae; Male; malondialdehyde; myeloperoxidase; nitric oxide; Plant Bark; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Rats; Rats, Wistar; TNF-α; Treatment Outcome; nitric oxide; myeloperoxidase; malondialdehyde; TNF-α; IL-1β; Anti-inflammatory
• ISSN: 1388-0209; 1744-5116
• DOI: 10.3109/13880209.2014.923005

Abstract Context: Careya arborea Roxb. (Lecythidaceae) has multiple applications in traditional medicine; it exhibits analgesic, antibacterial, anti-inflammatory, antiulcer, and protective effects. However, the effect of C. arborea on biochemical and immmunological inflammatory mediators has not been explored. Objective: The present study investigates the anti-inflammatory potential of the methanol extract of C. arborea stem bark and further assesses its possible mechanism on the modulation of inflammatory biomarkers. Materials and methods: Anti-inflammatory activity of C. arborea methanol extract (CAME) was evaluated (100 and 200 mg/kg, p.o) using indomethacin (10 mg/kg, p.o) as the standard drug in Wistar albino rats. Inflammation was induced by injecting 0.1 ml carrageenan (1% w/v) into the left hind paw. The anti-inflammatory mechanism was studied by measuring malondialdehyde (MDA), C-reactive protein (CRP), nitric oxide (NO), myeloperoxidase (MPO), TNF- , and IL-1β levels in both control and treated groups. A protocol has also been established to quantify quercetin and betulinic acid content in CAME using HPTLC fingerprint. Results: Careya arborea significantly (p < 0.001) decreased carrageenan-induced paw edema, showed a reduction of 48.87 and 65.53% at doses of 100 and 200 mg/kg, respectively. Moreover, CAME significantly decreased the MDA, CRP, NO, and MPO levels, elevated by carrageenan induced inflammation. CAME also markedly down-regulated serum TNF- and IL-1β levels. These findings were further supported by the histological study. The content of quercetin and betulinic acid in CAME was found to be 0.177 and 3.14%, respectively. Conclusion: Several mechanisms, including the inhibition of pro-inflammatory cytokines, enzymes and mediators release, appear to account for the anti-inflammatory potential of C. arborea.