Spleen immune status is affected after acute handling stress but not regulated by cortisol in Eurasian perch, Perca fluviatilis
The effects of acute stress on immune status and its regulation by cortisol/corticosteroid receptors have received little attention in percids. To address that question, we investigated the physiological and immune responses of Eurasian perch, Perca fluviatilis to acute stress. We exposed immature p...
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Published in | Fish & shellfish immunology Vol. 28; no. 5; pp. 931 - 941 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.05.2010
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The effects of acute stress on immune status and its regulation by cortisol/corticosteroid receptors have received little attention in percids. To address that question, we investigated the physiological and immune responses of Eurasian perch,
Perca fluviatilis to acute stress. We exposed immature perch to an 1-min exondation and measured at 1
h, 6
h, 24
h and 72
h post-stress: (1) stress-related parameters including plasma cortisol and glucose levels, (2) immune parameters in the plasma and in the spleen (complement, respiratory burst and lysozyme activity, total immunoglobulins; gene expression of lysozyme, complement unit 3, apolipoprotein A1 and 14
kDa, hepcidin and chemotaxin) (3) the corticosteroid receptors gene expression in the spleen after having cloned them. In addition, the
in vitro effects of cortisol on the spleen immune parameters were also investigated.
Plasma cortisol and glucose levels increased markedly 1
h post-stress and returned at basal levels after 24
h.
P. fluviatilis mineralocorticoid receptor, but not glucocorticoid receptors, was significantly up-regulated both
in vivo after the stress and
in vitro by cortisol at a physiological concentration (100
ng/ml). The plasma immune parameters were not significantly affected by the stress. In contrast, spleno-somatic index, spleen lysozyme activity, lysozyme and hepcidin gene expression were depleted and total immunoglobulins increased along the whole time-course (1–72
h). But, these immune parameters were not regulated
in vitro by cortisol at physiological or supra-physiological doses.
Our results indicate that handling stress may affect spleen antibacterial defences without clear effects on circulating immune compounds and that the elevation of plasma cortisol after handling stress may not be related to the regulation of this splenic response. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1050-4648 1095-9947 |
DOI: | 10.1016/j.fsi.2010.02.012 |