Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates

• Published in: Journal of microbiological methods Vol. 60; no. 2; pp. 275 - 279
• Main Authors: Couzinet, Sabine, Yugueros, Javier, Barras, Christine, Visomblin, Nathalie, Francois, Patrice, Lacroix, Bruno, Vernet, Guy, Lew, Daniel, Troesch, Alain, Schrenzel, Jacques, Jay, Corinne
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.02.2005; Elsevier Science
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Codon; DNA array; DNA Gyrase - genetics; DNA Topoisomerase IV - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Bacterial - genetics; Fluoroquinolone; Fluoroquinolones - pharmacology; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Mutation; Oligonucleotide Array Sequence Analysis - methods; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Polymerase Chain Reaction; Resistance; Staphylococcal Infections - drug therapy; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - metabolism; Resistance; Fluoroquinolone; DNA array; Staphylococcus aureus; High density; DNA chip; Characterization; Fluoroquinolone derivatives; Bacteria; Micrococcales; Micrococcaceae; Oligonucleotide; Mutation
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2004.09.015

Using high-density oligonucleotide array technology, 30 Staphylococcus aureus strains were studied for the presence of mutations in genes involved in fluoroquinolone resistance: grlA, gyrA, grlB and gyrB. For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%.