Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer

• Published in: World journal of gastroenterology : WJG Vol. 9; no. 11; pp. 2424 - 2427
• Main Authors: Xu, Ai-Hua, Chen, Hua-Sheng, Sun, Bu-Chan, Xiang, Xiao-Ren, Chu, Yun-Fei, Zhai, Fan, Jia, Ling-Chang
• Format: Journal Article
• Language: English
• Published: United States Medical College,Yangzhou University, Yangzhou 225001, Jiangsu Province, China%Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China%The First People Hospital of Yangzhou, Yangzhou 225002, Jiangsu Province, China%Jiangsu Provincial Subei People Hospital,Yangzhou 225001, Jiangsu Province, China 01.11.2003; Baishideng Publishing Group Inc
• Subjects: Administration, Oral; Adult; Aged; Aged, 80 and over; Apoptosis - drug effects; Cell Line, Tumor; Female; Flow Cytometry; Gastric Cancer; Ginkgo biloba; Humans; Male; Middle Aged; Phytotherapy; Plant Extracts - therapeutic use; Polysaccharides - therapeutic use; Proto-Oncogene Proteins c-bcl-2 - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Proto-Oncogene Proteins c-myc - metabolism; Stomach Neoplasms - drug therapy; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i11.2424

To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer. Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measured by electron gastroscope before and after treatment, then the inhibitory and effective rates were calculated. The ultrastructures of tumor cells were examined by transmissional electron microscope. Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of human gastric cancer SGC-7901 cells. Compared with the statement before treatment, GBEP capsules could reduce the area of tumors, and the effective rate was 73.4%. Ultrastructural changes of the cells indicated that GBEP could induce apoptosis and differentiation in tumor cells of patients with gastric cancer. GBEP could inhibit the growth of human gastric cancer SGC-7901 cells following 24-72 h treatment in vitro at 10-320 mg/L, which was dose- and time-dependent. GBEP was able to elevate the apoptosis rate and expression of c-fos gene, but reduce the expression of c-myc and bcl-2 genes also in a dose-dependent manner. The therapeutic mechanism of GBEP on human gastric cancer may relate to its effects on the expression of c-myc, bcl-2 and c-fos genes, which can inhibit proliferation and induce apoptosis and differentiation of tumor cells.