ETS2 function is required to maintain the transformed state of human prostate cancer cells

• Published in: Oncogene Vol. 17; no. 22; pp. 2883 - 2888
• Main Authors: SEMENTCHENKO, V. I, SCHWEINFEST, C. W, PAPAS, T. S, WATSON, D. K
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 03.12.1998; Nature Publishing Group
• Subjects: Agar; Androgens; Biological and medical sciences; Blotting, Northern; Cell Division; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cell Transformation, Neoplastic; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Ets-2 protein; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Neoplastic; Genes, Dominant; Humans; Male; Molecular and cellular biology; Phenotype; Phenotypes; Precipitin Tests; Prostate cancer; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Proto-Oncogene Protein c-ets-2; Proto-Oncogene Proteins - antagonists & inhibitors; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Repressor Proteins; RNA, Antisense - genetics; Sequence Deletion; Trans-Activators - antagonists & inhibitors; Trans-Activators - genetics; Trans-Activators - metabolism; Transcription Factors; Transfection; Tumor Cells, Cultured; Human; Cell function; Cell line; Prostate disease; Gene; Tumor progression; Tumor; Mutation; Gene expression; Cell transformation; Male genital diseases; Prostate
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1202220

The contribution of the ETS2 transcription factor to the transformed state in prostate cancer cells has been assessed. Northern blot analysis easily detects ETS2 in DU145 and PC3, high grade human prostate cell lines, but ETS2 is not present in lower grade LNCaP cells. Stable transfection of PC3 and DU145 prostate cell lines with an antisense ETS2 vector or with a dominant negative ETS2 mutant significantly reduced the ability of DU145 and PC3 cells to form large colonies in soft agar. Thus, the presence of ETS2 is positively correlated with a more transformed phenotype and blockage of ETS2 function can reduce transformed properties of prostate cancer cells.