Chronic granulomatous disease in two children with recurrent infections: Family studies using dihydrorhodamine-based flow cytometry

We employed a recently published technique, flow cytometry using the cell permeant dye dihydrorhodamine, to analyze families of two patients with X-linked chronic granulomatous disease. The results illustrate the utility of this method in the diagnosis of this serious immunodeficiency disease and al...

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Bibliographic Details
Published inThe Journal of pediatrics Vol. 130; no. 3; pp. 488 - 491
Main Authors Atkinson, T.Prescott, Bonitatibus, Gina M., Berkow, Roger L.
Format Journal Article
LanguageEnglish
Published New York, NY Mosby, Inc 01.03.1997
Elsevier
Subjects
Biological and medical sciences
Flow Cytometry - methods
Fluorescent Dyes
Genetic Carrier Screening
Genetic Linkage
Granulomatous Disease, Chronic - diagnosis
Granulomatous Disease, Chronic - genetics
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Infant
Male
Medical sciences
Neutrophils - drug effects
Tetradecanoylphorbol Acetate - pharmacology
X Chromosome
CGD
NBT
Chromosomal aberration
Human
Immunopathology
Flow cytometry
Abnormal X chromosome
Family study
Granulomatosis
Immune deficiency
Genetic disease
Chronic
Abnormal chromosome
Diagnosis
Technique
Child
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Summary:We employed a recently published technique, flow cytometry using the cell permeant dye dihydrorhodamine, to analyze families of two patients with X-linked chronic granulomatous disease. The results illustrate the utility of this method in the diagnosis of this serious immunodeficiency disease and also in the identification of carriers. (J Pediatr 1997;130:488-91)
ISSN:0022-3476
1097-6833
DOI:10.1016/S0022-3476(97)70218-X