Membrane fatty acid changes during the cell cycle of CV-1 cells

• Published in: Experimental cell research Vol. 191; no. 1; pp. 141 - 143
• Main Authors: Pan, D.A., Sullivan-Tailyour, G., Hulbert, A.J.
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.11.1990; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Cycle; Cell cycle, cell proliferation; Cell Line; Cell physiology; Chromatography, Gel; DNA - biosynthesis; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Membrane Lipids - metabolism; Molecular and cellular biology; Phospholipids - metabolism; G1» Phase; Cell line; Fatty acids; S Phase; Cell cycle; Plasma membrane
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(90)90047-E

Monolayers of CV-1 cells were synchronized at the G 1 S boundary of the cell cycle by a 24-h 2 m M thymidine blockade. Uptake of tritiated thymidine indicated that the peak DNA synthesis occurred 6–8 h after release from the block and that cell cycle time was 18–20 h. The fatty acid composition of phospholipids extracted from cells at 0, 7, and 18 h postblockade was measured by gas chromatography. The results indicate cyclic changes in membrane fatty acids with a significant increase in long-chain polyunsaturated fatty acids during the DNA synthesis phase (S phase) of the cell Cycle.