Emergence of imipenem resistance in Klebsiella pneumoniae owing to combination of plasmid-mediated CMY-4 and permeability alteration

Klebsiella pneumoniae BM2974 isolated from an abdominal abcess was resistant to high concentrations of all available β-lactams, including recently developed third-generation cephalosporins and carbapenems. Isoelectric focusing of β-lactamases and amplification, cloning and sequencing of the correspo...

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Published inJournal of antimicrobial chemotherapy Vol. 46; no. 6; pp. 895 - 900
Main Authors Cao, Van Thi Bao, Arlet, Guillaume, Ericsson, Britt-Marie, Tammelin, Ann, Courvalin, Patrice, Lambert, Thierry
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 01.12.2000
Oxford Publishing Limited (England)
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Summary:Klebsiella pneumoniae BM2974 isolated from an abdominal abcess was resistant to high concentrations of all available β-lactams, including recently developed third-generation cephalosporins and carbapenems. Isoelectric focusing of β-lactamases and amplification, cloning and sequencing of the corresponding genes, together with conjugation and transformation experiments, indicated that, in addition to the chromosomally encoded β-lactamase, the strain produced three plasmid-mediated β-lactamases with pIs of 5.4, 8.2 and 9.0, which corresponded to TEM-1, SHV-5 and AmpC-type CMY-4, respectively. Strain BM2974 also lacked a major outer membrane protein of c. 40 kDa which was present in the spontaneous imipenem-susceptible revertant BM2974-1. We suggest that imipenem resistance in strain BM2974 is attributable to production of CMY-4 β-lactamase combined with permeability alteration.
Bibliography:istex:56C10390E15BAF7A74F94CA13D53EEEC8433A527
PII:1460-2091
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SourceType-Scholarly Journals-1
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content type line 23
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/46.6.895