Emergence of imipenem resistance in Klebsiella pneumoniae owing to combination of plasmid-mediated CMY-4 and permeability alteration
Klebsiella pneumoniae BM2974 isolated from an abdominal abcess was resistant to high concentrations of all available β-lactams, including recently developed third-generation cephalosporins and carbapenems. Isoelectric focusing of β-lactamases and amplification, cloning and sequencing of the correspo...
Saved in:
Published in | Journal of antimicrobial chemotherapy Vol. 46; no. 6; pp. 895 - 900 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.12.2000
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Klebsiella pneumoniae BM2974 isolated from an abdominal abcess was resistant to high concentrations of all available β-lactams, including recently developed third-generation cephalosporins and carbapenems. Isoelectric focusing of β-lactamases and amplification, cloning and sequencing of the corresponding genes, together with conjugation and transformation experiments, indicated that, in addition to the chromosomally encoded β-lactamase, the strain produced three plasmid-mediated β-lactamases with pIs of 5.4, 8.2 and 9.0, which corresponded to TEM-1, SHV-5 and AmpC-type CMY-4, respectively. Strain BM2974 also lacked a major outer membrane protein of c. 40 kDa which was present in the spontaneous imipenem-susceptible revertant BM2974-1. We suggest that imipenem resistance in strain BM2974 is attributable to production of CMY-4 β-lactamase combined with permeability alteration. |
---|---|
Bibliography: | istex:56C10390E15BAF7A74F94CA13D53EEEC8433A527 PII:1460-2091 ark:/67375/HXZ-GLC3LB7H-D local:0460895 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0305-7453 1460-2091 1460-2091 |
DOI: | 10.1093/jac/46.6.895 |