Aeromonas tecta sp. nov., isolated from clinical and environmental sources

• Published in: Systematic and applied microbiology Vol. 31; no. 4; pp. 278 - 286
• Main Authors: Demarta, Antonella, Küpfer, Mara, Riegel, Philippe, Harf-Monteil, Colette, Tonolla, Mauro, Peduzzi, Raffaele, Monera, Arturo, José Saavedra, Maria, Martínez-Murcia, Antonio
• Format: Journal Article
• Language: English
• Published: München Elsevier GmbH 01.09.2008; Elsevier
• Subjects: 16S rRNA; Adult; Aeromonas; Aeromonas - classification; Aeromonas - drug effects; Aeromonas - genetics; Aeromonas - isolation & purification; Aeromonas encheleia; Aeromonas tecta sp. nov; Animals; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; Child; DNA Gyrase - genetics; DNA-Directed RNA Polymerases - genetics; DNA–DNA hybridization; Environmental Microbiology; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Glycerol - metabolism; gyrB; Humans; Microbial Sensitivity Tests; Microbiology; Oncorhynchus mykiss - microbiology; Phylogeny; RNA, Ribosomal, 16S - genetics; rpoD; Sigma Factor - genetics; Species Specificity; Systematics; Taxonomy; 16S rRNA; gyrB; Aeromonas tecta sp. nov; Taxonomy; rpoD; Phylogeny; DNA–DNA hybridization; Aeromonas; Hybridization; DNA-DNA hybridization; DNA; 16S-RNA; Bacteria; Vibrionaceae; Environment; New species
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2008.04.005

Five Aeromonas strains, isolated from both clinical and environmental sources and characterized by a polyphasic approach, including phylogenetic analysis derived from gyrB, rpoD, and 16S rRNA gene sequencing, as well as DNA–DNA hybridization, extensive biochemical and antibiotic susceptibility tests, were recognized as members of an unknown, or undescribed, Aeromonas species. These “ Aeromonas eucrenophila-like” strains were closely related to the species A. eucrenophila and Aeromonas encheleia, but they were negative for indole and acid from glycerol tests. Therefore, based on the results of the phylogenetic analyses and DNA–DNA pairing data of these strains, a novel species of the genus Aeromonas is described, for which the name Aeromonas tecta is proposed with isolate F518 T (CECT7082 T, DSM17300 T, MDC91 T) as the type strain.