Comparison of Culture- and Quantitative PCR-Based Indicators of Antibiotic Resistance in Wastewater, Recycled Water, and Tap Water

• Published in: International journal of environmental research and public health Vol. 16; no. 21; p. 4217
• Main Authors: Rocha, Jaqueline, Fernandes, Telma, Riquelme, Maria V, Zhu, Ni, Pruden, Amy, Manaia, Célia M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.10.2019; MDPI
• Subjects: Activated carbon; Activated sludge; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Bacteria; Cell culture; Cellulose; Cellulose nitrate; Coliforms; Deoxyribonucleic acid; Disinfection; Disinfection & disinfectants; DNA; Drinking water; Drinking Water - microbiology; Drug resistance; Drug Resistance, Microbial - genetics; Effluents; Fecal coliforms; Feces; Gene expression; Genes, Bacterial; Humans; Laboratories; Membranes; Methods; Municipal water supplies; Phenotypes; Polymerase Chain Reaction; Porosity; Portugal; Reclaimed water; Recycling; Sludge treatment; Software; Tertiary treatment; Ultraviolet radiation; United States; Variance analysis; Waste Water - microbiology; Wastewater treatment; Wastewater treatment plants; Water - analysis; Water distribution; Water distribution systems; Water engineering; Water Microbiology; Water purification; Water quality; Water reuse; Water supply; Water treatment; United States; Portugal; United States > US; antibiotic resistance monitoring; water quality; antibiotic resistant coliforms
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph16214217

Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for gene (0%-57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with and 1 (0.725 ≤ R ≤ 0.762; < 0.0001). Further, presumptive total and fecal coliforms correlated with the -specific biomarkers, and , suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides ( and ) were the most abundant, followed by genes encoding resistance to tetracyclines ( (A) and (O)) and β-lactams ( and ), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment.