Production and characterisation of antibodies to vanillylmandelic acid

• Published in: Journal of immunological methods Vol. 118; no. 1; p. 101
• Main Authors: Mellor, G W, Gallacher, G, Landon, J
• Format: Journal Article
• Language: English
• Published: Netherlands 10.03.1989
• Subjects: Animals; Antibody Specificity; Chemical Phenomena; Chemistry; Sheep; Structure-Activity Relationship; Vanilmandelic Acid - analogs & derivatives; Vanilmandelic Acid - immunology

Antibodies were raised to the catecholamine metabolite vanillylmandelic acid (VMA). The side chain was protected and, after derivatisation through the 4-phenolic hydroxyl group, the hapten was coupled to KLH and the resultant conjugate deprotected. Sheep immunised with this immunogen responded with...