Production and characterisation of antibodies to vanillylmandelic acid

Antibodies were raised to the catecholamine metabolite vanillylmandelic acid (VMA). The side chain was protected and, after derivatisation through the 4-phenolic hydroxyl group, the hapten was coupled to KLH and the resultant conjugate deprotected. Sheep immunised with this immunogen responded with...

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Bibliographic Details
Published inJournal of immunological methods Vol. 118; no. 1; p. 101
Main Authors Mellor, G W, Gallacher, G, Landon, J
Format Journal Article
LanguageEnglish
Published Netherlands 10.03.1989
Subjects
Animals
Antibody Specificity
Chemical Phenomena
Chemistry
Sheep
Structure-Activity Relationship
Vanilmandelic Acid - analogs & derivatives
Vanilmandelic Acid - immunology
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Summary:Antibodies were raised to the catecholamine metabolite vanillylmandelic acid (VMA). The side chain was protected and, after derivatisation through the 4-phenolic hydroxyl group, the hapten was coupled to KLH and the resultant conjugate deprotected. Sheep immunised with this immunogen responded with specific, high titre antibodies to VMA as assessed using a fluorescent label. Cross-reaction of the antiserum from one of the sheep was minimal, being under 1% with all naturally occurring related compounds tested. This report is the first to describe an effective antiserum for VMA which will permit measurement over the normal range in urine.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(89)90059-8