The trans-10,cis-12 isomer of conjugated linoleic acid reduces hepatic triacylglycerol content without affecting lipogenic enzymes in hamsters

• Published in: British journal of nutrition Vol. 92; no. 3; pp. 383 - 389
• Main Authors: Zabala, Amaia, Churruca, Itziar, Macarulla, M. Teresa, Rodríguez, Víctor M., Fernández-Quintela, Alfredo, Martínez, J. Alfredo, Portillo, María P.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.09.2004
• Subjects: Acetyl-CoA Carboxylase - metabolism; Animals; Biological and medical sciences; Blood Glucose - analysis; Blood Glucose - metabolism; Body fat; CCAAT-Enhancer-Binding Proteins - metabolism; Conjugated linoleic acid; Cricetinae; Diet; DNA-Binding Proteins - metabolism; Enzymes; Fatty Acid Synthases - metabolism; Fatty acids; Feeding. Feeding behavior; Food; Fundamental and applied biological sciences. Psychology; Gene expression; Hamster; Insulin - blood; Isomerism; Laboratory animals; Linoleic Acids, Conjugated - administration & dosage; Linoleic Acids, Conjugated - pharmacology; Lipid Metabolism; Lipids; Lipogenesis; Liver; Liver - metabolism; Male; Mesocricetus; Metabolism; Nutrition; Proteins; Sterol regulatory element binding protein; Sterol Regulatory Element Binding Protein 1; Sterols; Transcription Factors - metabolism; Triglycerides - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems; United States > US; Spain; Conjugated linoleic acid; Sterol regulatory element binding protein; Lipogenesis; Liver; Hamster; Vertebrata; Mammalia; Triacylglycerol; Digestive system; Enzyme; Animal; Rodentia; Conjugated linoleic acid: Lipogenesis: Sterol regulatory element binding protein: Liver: Hamster; Isomer; Linoleic acid
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN20041220

Conjugated linoleic acid (CLA) refers to the positional and geometric dienoic isomers of linoleic acid. The dietary intake of CLA has been associated with changes in lipid metabolism. The aim of the present work was to assess the effects of the two main isomers of CLA on sterol regulatory element binding protein (SREBP)-1a and SREBP-1c mRNA levels, as well as on mRNA levels and the activities of several lipogenic enzymes in liver. For this purpose hamsters were fed an atherogenic diet supplemented with 5 g linoleic acid, cis-9,trans-11 or trans-10,cis-12 CLA/kg diet for 6 weeks. The trans-10,cis-12 isomer intake produced significantly greater liver weight, but also significantly decreased liver fat accumulation. No changes in mRNA levels of SREBP-1a, SREBP-1c and lipogenic enzymes, or in the activities of these enzymes, were observed. There was no effect of feeding cis-9,trans-11 CLA. These results suggest that increased fat accumulation in liver does not occur on the basis of liver enlargement produced by feeding the trans-10,cis-12 isomer of CLA in hamsters. The reduction in hepatic triacylglycerol content induced by this isomer was not attributable to changes in lipogenesis.