Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells

• Published in: Journal of natural medicines Vol. 66; no. 1; pp. 49 - 54
• Main Authors: Yayeh, Taddesse, Oh, Won Jun, Park, Seung-Choon, Kim, Tae-Hwan, Cho, Jae Youl, Park, Hwa-Jin, Lee, In-Kyoung, Kim, Sang-Keun, Hong, Seung-Bok, Yun, Bong-Sik, Rhee, Man Hee
• Format: Journal Article
• Language: English
• Published: Japan Springer-Verlag 2012; Springer Japan
• Subjects: Acetates - chemistry; Agaricales - chemistry; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Biomedical and Life Sciences; Biomedicine; Blotting, Western; Cell Line; Complementary & Alternative Medicine; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Cytokines - metabolism; cytotoxicity; Dinoprostone - metabolism; Dose-Response Relationship, Drug; enzyme immunoassays; ethyl acetate; gene expression; Gene Expression Regulation, Enzymologic; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism; human diseases; Immunoenzyme Techniques; Inflammation Mediators - metabolism; Interleukin-1beta - metabolism; interleukin-6; Interleukin-6 - metabolism; lipopolysaccharides; Lipopolysaccharides - pharmacology; macrophage colony-stimulating factor; macrophages; Macrophages - drug effects; Macrophages - enzymology; Macrophages - immunology; Medicinal Chemistry; Medicine, Traditional; messenger RNA; Mice; mushrooms; nitric oxide; Nitric Oxide - metabolism; nitric oxide synthase; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Original Paper; Pharmacology/Toxicology; Pharmacy; Phellinus; Plant Sciences; prostaglandin synthase; prostaglandins; protein synthesis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Solvents - chemistry; traditional medicine; Western blotting; Inflammation; Inflammatory mediator; Proinflammatory cytokine; Macrophage
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-011-0552-8

Mushrooms are valuable sources of biologically active compounds possessing anticancer, antiplatelet, and anti-inflammatory properties. Phellinus baumii is a mushroom used in folk medicine for a variety of human diseases. However, its potential anti-inflammatory effect has remained unclear. Therefore, we studied the effect of P. baumii ethyl acetate extract (PBEAE) on inflammatory mediator and proinflammatory cytokine protein and/or mRNA expression levels using the nitric oxide (NO) assay, enzyme immunoassay (EIA), western blot, and reverse transcription polymerase chain reaction (RT–PCR) in lipopolysaccharide (LPS)-stimulated macrophage like RAW264.7 cells. PBEAE markedly inhibited NO generation and prostaglandin E2 (PGE2) synthesis in a concentration-dependent pattern without any cytotoxic effect at the concentration range used. PBEAE also suppressed inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. In addition, LPS-induced iNOS and COX-2 mRNA expression levels were dose-dependently inhibited by PBEAE pretreatment. Furthermore, PBEAE attenuated the mRNA expression levels of proinflammatory cytokines, specifically interleukin (IL)-1β, IL-6, and granulocyte macrophage colony-stimulating factor (GM-CSF), in a concentration-dependent fashion. Our study suggests that P. baumii might exhibit anti-inflammatory properties by downregulating proinflammatory mediators. Thus, further study on compounds isolated from PBEAE is warranted to investigate the associated molecular mechanisms and identify the potential therapeutic targets.