The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline–Alkali Stress

• Published in: Agronomy (Basel) Vol. 13; no. 9; p. 2343
• Main Authors: Wang, Qiang, Liang, Xiaotian, Xiang, Dabing, Xu, Weiwei, Wang, Chunlong, Zhan, Chao, Ren, Changzhong, Wei, Liming, Zhang, Shuqiao, Zhang, Li, Wang, Junying, Guo, Laichun
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.09.2023
• Subjects: Abiotic stress; Abscisic acid; Aluminum; Analysis; Biosynthesis; Carbon dioxide; Carbon dioxide concentration; Carotenoids; Cell division; Chlorophyll; Cluster analysis; Copper; Energy absorption; Germination; Homeostasis; Iron; Leaves; Magnesium; Manganese; Melatonin; Metabolism; Molybdenum; Morphogenesis; oat; Oats; Photosynthesis; physiological biochemistry; Physiology; Plant growth; Plant resistance; Plant tolerance; Product development; Root development; saline–alkali; Salt; Seed germination; seedling growth; Seedlings; Seeds; Senescence; Titanium; Transpiration; Tryptamine; Tryptamines; Massachusetts; China; Beijing China; Jilin China
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy13092343

Exogenous melatonin (MT) regulates plant growth and mitigates stress in response to stress. To analyze the machinery of exogenous melatonin, which improves salt and alkaline tolerance in oats, MT’s function was identified in the oat seed germination stage in our previous study. In this study, morphogenesis, photosynthetic physiology, hormone levels, and ion homeostasis were evaluated using the same MT treatment concentration. The results revealed that compared to the S45 treatment, the 100 μmol·L−1 MT treatment efficiently increased the seedling height and main root length of oat seedlings; promoted secondary root development; enhanced the root volume and root surface area; maintained a higher photosynthetic pigment content (carotenoids; chlorophyll a; chlorophyll b); raised the leaf photosynthetic rate (Pn), intercellular CO2 concentration (Ci), conductance to H2O (Gs), and transpiration rate (Tr); enhanced the light energy absorption and conversion of leaves; increased the leaf GA3, Tryptamine (TAM), and IAA contents; and decreased ABA levels. Hierarchical cluster analysis revealed that MT treatment also increased the contents of P, K, Ca, Mn, Cu, Mg, Fe, Zn, Mo, Cd, Al, Se, Ni, Co, and Ti; decreased the Na/K ratio; and maintained cellular ionic homeostasis in oat seedlings under saline–alkali stress, as compared with the untreated group. These findings showed that MT treatment enhanced the adaptation of oat to saline–alkali stress through regulating the physiological process of seedling growth. This suggests that MT plays a different role in improving saline–alkali tolerance in the germination and seedling stages of oat.