Determination of succinylcholine in human plasma by high-performance liquid chromatography with electrochemical detection

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 718; no. 1; pp. 129 - 134
• Main Authors: Gao, H., Roy, S., Donati, F., Varin, F.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.10.1998; Elsevier Science
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; General pharmacology; Humans; Medical sciences; Neuromuscular Depolarizing Agents - blood; Pharmacology. Drug treatments; Reference Standards; Sensitivity and Specificity; Succinylcholine; Succinylcholine - blood; Succinylcholine; Depolarisant myorelaxant; Human; Biological fluid; Solid phase extraction; Suxamethonium chloride; HPLC chromatography; Quantitative analysis; Electrochemical detector; Blood plasma
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(98)00350-8

An alternative HPLC method for the quantification of the depolarizing neuromuscular blocking agent succinylcholine in human plasma is described. Drug spiked plasma and patient plasma samples were extracted using a C 1 solid-phase cartridge. Succinylcholine was separated on a Cyano column and quantitated using electrochemical detection at a potential of 450 mV and 750 mV. Mobile phase consisted of a mixture of phosphoric acid–acetonitrile–methanol (45:35:25) adjusted to an apparent pH of 5. Standard curves for the quantitation were linear in the range of 250–8000 ng/ml. Between-day and within-day relative standard deviations were 5.1% and 1.7%, respectively. Mean drug recovery and accuracy was 68% and 104%, respectively.