CRISPR-Cas9 Mediated Genome Editing in Drosophila

In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in ....

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Published inBio-protocol Vol. 9; no. 2; p. e3141
Main Authors Peng, Ping, Wang, Xia, Shen, Da, Sun, Jin, Jia, Yu, Xu, Rong-Gang, Zhu, Li-Fei, Ni, Jian-Quan
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.01.2019
Bio-protocol LLC
Subjects
Biology
Methods
Heritable mutations
CRISPR
Genome editing
Cas9
Drosophila
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Summary:In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in . In this optimized system, the mutant progeny can be generated by only injecting a DNA plasmid encoding synthetic guide RNA (sgRNA) under the control of the U6b promoter into transgenic fly embryos in which Cas9 is specifically expressed in the progenitor cells, thus the gene of interest can be edited by the CRISPR in germ cells, with high rate of heritable mutations and few side effects.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3141