Changes in pulmonary and plasma oxidative stress and inflammation following eccentric and concentric cycling in stable COPD patients

• Published in: European journal of applied physiology Vol. 121; no. 6; pp. 1677 - 1688
• Main Authors: Valladares-Ide, Denisse, Bravo, Maria José, Carvajal, Ana, Araneda, Oscar F., Tuesta, Marcelo, Reyes, Alvaro, Peñailillo, Reyna, Peñailillo, Luis
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2021; Springer Nature B.V
• Subjects: Antioxidants; Biomedical and Life Sciences; Biomedicine; Chronic obstructive pulmonary disease; Creatine; Creatine kinase; DRKS; DRKS00009755; Glutathione peroxidase; Human Physiology; Hydrogen peroxide; Inflammation; Interleukin 6; Lung diseases; Malondialdehyde; Muscle strength; Nitrites; Nitrogen dioxide; Obstructive lung disease; Occupational Medicine/Industrial Medicine; Original Article; Oxidative stress; pH effects; Sports Medicine; Tumor necrosis factor-α; Vitamin E; TBARS; TNF-α; Exhaled breath condensate; Lengthening; COPD; Interleukin-6
• ISSN: 1439-6319; 1439-6327
• DOI: 10.1007/s00421-021-04652-1

Purpose The purpose of this study was to compare pulmonary and plasma markers of oxidative stress and inflammation after concentric and eccentric cycling bouts in individuals with chronic obstructive pulmonary disease (COPD). Methods Ten patients with moderate COPD level (68.3 ± 9.1 years; forced expiratory volume in 1 s = 68.6 ± 20.4% of predicted) performed 30 min of moderate-intensity concentric (CONC-M: 50% maximum concentric cycling power output; PO max ) and eccentric cycling (ECC-M: 50% PO max ), and high-intensity eccentric cycling (ECC-H: 100% PO max ) in a randomised order. Cardiometabolic demand was monitored during cycling. Indirect markers of muscle damage were assessed before, immediately after, 24 and 48 h after cycling (muscle strength, muscle soreness and creatine kinase activity). Plasma oxidative stress (malondialdehyde: MDA), antioxidant (glutathione peroxidase activity: GPx) and inflammatory markers (IL-6, TNF-α) were measured before and 5 min after cycling. Exhaled breath condensate (EBC) samples were collected before and 15 min after cycling and analysed for hydrogen peroxide (H 2 O 2 ), nitrites (NO 2− ) and pH . Results Cardiometabolic demand was 40–50% lesser for ECC-M than CONC-M and ECC-H. Greater muscle damage was induced after ECC-H than ECC-M and CONC-M. MDA decreased immediately after CONC-M (− 28%), ECC-M (− 14%), and ECC-H (− 17%), while GPx remained unchanged. IL-6 increased only after ECC-H (28%), while TNF-α remained unchanged after exercise. Pulmonary H 2 O 2 , NO 2− and pH remained unchanged after exercise. Conclusion These results suggest that only moderate muscle damage and inflammation were induced after high-intensity eccentric cycling, which did not induce pulmonary or plasmatic increases in markers of oxidative stress. Trial registration number Trial registration number: DRKS00009755.