Correlation between Salivary Levels of IGF-1, IGFBP-3, IGF-1/IGFBP3 Ratio with Skeletal Maturity Using Hand-Wrist Radiographs

• Published in: International journal of environmental research and public health Vol. 19; no. 6; p. 3723
• Main Authors: Almalki, Abdullah, Thomas, Julie Toby, Khan, Abdul Rehman Ahmed, Almulhim, Basim, Alassaf, Abdullah, Alghamdi, Sara Ayid, Joseph, Betsy, Alqerban, Ali, Alotaibi, Saud
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.03.2022; MDPI
• Subjects: Adolescent; Adult; Age; Biomarkers; Child; Coronaviruses; COVID-19; Cross-Sectional Studies; Dentistry; Enzymes; Female; Growth hormones; Humans; Insulin; Insulin-Like Growth Factor Binding Protein 3; Insulin-Like Growth Factor I - metabolism; Insulin-like growth factors; Investigations; Kruskal-Wallis test; Male; Orthodontics; Radiography; Sample size; Vertebrae; Wrist; Young Adult; Saudi Arabia; United States > US; orthodontics; insulin-like growth factor; hand wrist radiographs; salivary diagnostics; skeletal maturity; insulin-like growth factor binding protein
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph19063723

Objective: The relevance of growth determination in orthodontics is driving the search for the most precise and least invasive way of tracking the pubertal growth spurt. Our aim was to explore whether minimally invasive salivary estimation of biomarkers Insulin-like growth factor (IGF-1) and Insulin-like growth factor binding protein-3 (IGFBP-3) could be used to estimate skeletal maturity for clinical convenience, especially in children and adolescent age groups. Materials and Method: The cross-sectional study was conducted on 90 participants (56 girls and 34 males) with ages ranging from 6 to 25 years. Each subject’s hand-wrist radiograph was categorized based on skeletal maturity, and saliva samples were estimated for IGF-1 and IGFBP-3 using the respective ELISA kits. Kruskal–Wallis nonparametric ANOVA was applied to compare different skeletal stages. Results: The study demonstrated low salivary IGF-1 levels at the prepubertal stage, with increase during pubertal onset and peak pubertal stage followed by a decline during pubertal deceleration to growth completion. Spearman’s correlation coefficient demonstrated a strong positive association (r = 0.98 p < 0.01) between salivary IGF/IGFBP-3 ratio and different stages of skeletal maturity. Conclusion: Salivary IGF-1, IGFBP-3, and IGF/IGFBP-3 ratio could serve as a potential biochemical marker for predicting the completion of skeletal maturity.