Stimulation of renal Na + dicarboxylate cotransporter 1 by Na +/H + exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B

Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na +/H + exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid induci...

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Published inBiochemical and biophysical research communications Vol. 313; no. 4; pp. 998 - 1003
Main Authors Boehmer, Christoph, Embark, Hamdy M, Bauer, Anna, Palmada, Monica, Yun, Chris H, Weinman, Edward J, Endou, Hitoshi, Cohen, Philip, Lahme, Sven, Bichler, Karl-Horst, Lang, Florian
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 23.01.2004
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Summary:Renal tubular citrate transport is accomplished by electrogenic Na + coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na +/H + exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B ( T308,S473DPKB). Succinate induced inward currents ( I succ) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered I succ. On the other hand, coexpression of SGK1, SGK3, and T308,S473DPKB stimulated I succ, an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H + secretion.
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ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2003.12.011