Identification of DNA-protein interactions and enhancer activity at the 5′ end of the upstream regulatory region in human papillomavirus type 11

• Published in: Virology (New York, N.Y.) Vol. 170; no. 1; pp. 123 - 130
• Main Authors: Auborn, K.J., Galli, R.L., Dilorenzo, T.P., Steinberg, B.M.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.1989; Elsevier
• Subjects: Antigens, Viral, Tumor - genetics; Biological and medical sciences; Cells, Cultured; Cloning, Molecular; DNA-Binding Proteins - physiology; Enhancer Elements, Genetic; Fundamental and applied biological sciences. Psychology; Genetics; Laryngeal Neoplasms - genetics; Larynx - physiology; Microbiology; Papilloma - genetics; Papillomaviridae - genetics; Protein Binding; Regulatory Sequences, Nucleic Acid; Virology; Nucleotide sequence; Gel electrophoresis; DNA binding protein; Molecular interaction; Binding site; Papovaviridae; Papillomavirus; Virus; Proteins; Human papillomavirus; Regulation(control); Enhancer sequence; DNA
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(89)90359-0

We have examined the 5′ end of the noncoding region of the genome of a human papillomavirus, HPV-11, for regulatory elements using permissive host cells. This region of unknown function in the upstream regulatory region (URR) is known to have unusual DNA structure and frequently contains rearrangements which are associated with some more virulent isolates. This 5′ 269-bp fragment was found to exhibit both specific DNA-protein binding using laryngeal papilloma protein extracts and enhancer activity in normal and papillornatous primary laryngeal cells. The viral DNA flanks the Li open reading frame and does not contain the viral E2 binding site. Three distinct protein binding sites are contained in a 50-bp region of the fragment. This fragment, as a whole, functions as an enhancer in primary laryngeal and papilloma cells when ligated to the SV40 promoter and SV40 T-antigen gene. We conclude that this part of the noncoding region of the papillomaviruses has elements characteristic of regulatory elements in cells permissive for infection by these viruses.