Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant
During leaf development, the timing of transition from cell proliferation to expansion is an important factor in determining the final organ size. However, the regulatory system involved in this transition remains less understood. To get an insight into this system, we investigated the compensation...
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Published in | Frontiers in plant science Vol. 15; p. 1322223 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.04.2024
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Subjects | |
Online Access | Get full text |
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Summary: | During leaf development, the timing of transition from cell proliferation to expansion is an important factor in determining the final organ size. However, the regulatory system involved in this transition remains less understood. To get an insight into this system, we investigated the compensation phenomenon, in which the cell number decreases while the cell size increases in organs with determinate growth. Compensation is observed in several plant species suggesting coordination between cell proliferation and expansion. In this study, we examined an Arabidopsis mutant of
(
)/
, a positive regulator of cell proliferation, which exhibits the compensation. Though the AN3 role has been extensively investigated, the mechanism underlying excess cell expansion in the
mutant remains unknown. Focusing on the early stage of leaf development, we performed kinematic, cytological, biochemical, and transcriptome analyses, and found that the cell size had already increased during the proliferation phase, with active cell proliferation in the
mutant. Moreover, at this stage, chloroplasts, vacuoles, and xylem cells developed earlier than in the wild-type cells. Transcriptome data showed that photosynthetic activity and secondary cell wall biosynthesis were activated in
proliferating cells. These results indicated that precocious cell differentiation occurs in
cells. Therefore, we suggest a novel AN3 role in the suppression of cell expansion/differentiation during the cell proliferation phase. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Michael Lenhard, University of Potsdam, Germany Reviewed by: Fabrice Besnard, UMR5667 Laboratoire Reproduction et Développement des Plantes (RDP), France Michael Gerard Muszynski, University of Hawaii at Manoa, United States Edited by: Neelima Roy Sinha, University of California, Davis, United States Present address: Kazune Ezaki, Department of Life Science, Rikkyo University, Toshima-ku, Tokyo, Japan |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2024.1322223 |