Evidence for Decreased Expression of ADAMTS-1 Associated With Impaired Oocyte Quality in PCOS Patients
Context: Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may b...
Saved in:
Published in | The journal of clinical endocrinology and metabolism Vol. 99; no. 6; pp. E1015 - E1021 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Endocrine Society
01.06.2014
Copyright by The Endocrine Society |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Context:
Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may be altered in PCOS granulosa cell (GC)-reflecting abnormalities in ovulatory signaling.
Objective:
The purpose of this paper is to analyze the differential expression of ADAMTS-1 in PCOS patients associated with impaired oocyte quality.
Design and Setting:
A prospective comparative experimental study was performed at a clinical reproductive medicine center.
Patients:
Women with PCOS (n = 40) and normovulatory controls (n = 40) undergoing controlled ovarian hyperstimulation and in vitro fertilization were recruited in our study.
Main Outcome Measures:
Differential expression of ADAMTS-1 in GCs was analyzed with immunocytochemistry in PCOS patients and normal controls, and ADAMTS-1 mRNA expression was quantified by RT-PCR. Furthermore, the correlation between ADAMTS-1 mRNA and oocyte quality was analyzed.
Results:
The expression of ADAMTS-1 was decreased in PCOS patients compared with normally ovulating women and was closely related to lower oocyte recovery, oocyte maturity, and fertilization rate.
Conclusion:
Our study provides evidence that the dysregulated expression of ADAMTS-1 in PCOS may influence oocyte quality-via GCs-oocyte paracrine and endocrine mechanism. |
---|---|
Bibliography: | The work was supported by grants from the National Natural Science Foundation of China (Grant 81100470), the Young Teacher Training Project of Sun Yat-Sen University (Grant 12ykpy24), and the Key Laboratory of Reproductive Medicine of Guangdong Province (Grant 2012A06140003). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-972X 1945-7197 |
DOI: | 10.1210/jc.2013-4177 |