Tumor necrosis factor alpha and interleukin 1 alpha enhance lipopolysaccharide-mediated bovine endothelial cell injury

• Published in: Journal of leukocyte biology Vol. 51; no. 6; pp. 579 - 585
• Main Authors: Sharma, Smita A., Olchowy, Timothy W.J., Yang, Zhengang, Breider, Mike A.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Society for Leukocyte Biology 01.06.1992; Federation of American Societies for Experimental Biology
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; bovine; Cattle; Cells, Cultured; Drug Synergism; endothelial; Endothelium, Vascular - drug effects; Endothelium, Vascular - pathology; endotoxin; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; interleukin 1; Interleukin-1 - pharmacology; lipopolysaccharide; Lipopolysaccharides - toxicity; macrophage; Macrophages, Alveolar - physiology; Miscellaneous; Pasteurella haemolytica; receptor antagonist; Regulatory factors and their cellular receptors; tumor necrosis factor; Tumor Necrosis Factor-alpha - pharmacology; Endothelial cell; Bovine; Lung; Cytokine; Interleukin 1α; Cytotoxicity; Synergism; Pasteurellaceae; Vertebrata; Mixed cell culture; Mammalia; Pulmonary alveolus; Lipopolysaccharide; Bacteria; Models; Artiodactyla; Mechanism of action; Ungulata; Tumor necrosis factor α; Pasteurella haemolytica; Macrophage
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.51.6.579

Alveolar macrophages (AMs) are important in the host response to aerogenous pulmonary bacterial infections, such as Pasteurella haemolytica–induced pneumonia in cattle. Previous work has shown that AMs enhance P. haemolytica–mediated pulmonary endothelial cell (EC) damage in vitro. The purpose of this study was to determine the mechanism of AM‐enhanced EC damage using an in vitro AM‐EC coculture system consisting of AMs cultured on culture plate insert membranes and ECs in the underlying chamber. The addition of lipopolysaccharide (LPS) to the culture plate insert chamber resulted in EC damage indicated by 51Cr release, which was enhanced in the presence of AMs. To determine the role of AM‐secreted cytokines, recombinant human interleukin 1 α (IL‐1) or tumor necrosis factor α (TNF) was added to ECs simultaneously with varying concentrations of LPS. Although TNF and IL‐1 alone had only marginal toxic effects on ECs, the simultaneous treatment of TNF or IL‐1 with LPS greatly increased the LPS cytotoxic effect on ECs. In addition, IL‐1 receptor antagonist eliminated the IL‐1 enhancement of LPS‐mediated EC toxicity. These results suggest that macrophage‐secreted cytokines synergistically enhance LPS‐mediated pulmonary EC damage.