CELL TYPE-DEPENDENT COLLAGEN-TYPE RECOGNITION BY CELL RECEPTORS

Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tiss...

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Published inCell biology international Vol. 25; no. 7; pp. 643 - 648
Main Authors Sadofiev, L.A., Nikolaenko, N.S., Kalmykova, N.V., Podgornaya, O.I.
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier Ltd 01.07.2001
Blackwell Publishing Ltd
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Summary:Affinity chromatography of a number of cell types on collagens I and III reveals three proteins with MRof 250, 170 and 140kDa. These proteins are able to discriminate between types I and III, but not types III and IV. Collagen-type recognition is therefore characteristic for cells of connective tissue origin. Polyclonal antibodies (Ab) raised against 170 and 140kDa polypeptides and used in immunofluorescence show membrane localisation for both, with their distribution being similar to each other and to the distribution of the integrin β1 chain. Abp140 and commercial monoclonal antibodies against α2chain stain a band of the same molecular mass as from purified collagen binding proteins from liver cells, indicating that the 140kDa protein is probably the α2integrin chain. The α2chain containing integrins are therefore able to discriminate collagen types I and III and collagen type recognition by this receptor is cell-type dependent.
Bibliography:ark:/67375/WNG-V4767M9W-F
istex:5B1F032EA12F86FD4A9F2F3D8A36D032CB9A412B
ArticleID:CBIN1157
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.1999.0487