Neutrophil membrane sulfhydryl groups are involved in stimulated neutrophil adherence to endothelium
We investigated the role of membrane sulfhydryl groups in adherence of stimulated polymorphonuclear neutrophils to cultured endothelial cells. Treatment of neutrophils with p‐chloromercuriphenyl sulfonate (PCMPS), a slowly penetrating sulfhydryl reagent, inhibited phorbol myristate acetate (PMA)‐ or...
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Published in | Journal of leukocyte biology Vol. 45; no. 3; pp. 177 - 182 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
Society for Leukocyte Biology
01.03.1989
Federation of American Societies for Experimental Biology |
Subjects | |
Online Access | Get full text |
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Summary: | We investigated the role of membrane sulfhydryl groups in adherence of stimulated polymorphonuclear neutrophils to cultured endothelial cells. Treatment of neutrophils with p‐chloromercuriphenyl sulfonate (PCMPS), a slowly penetrating sulfhydryl reagent, inhibited phorbol myristate acetate (PMA)‐ or calcium ionophore A23187‐stimulated adherence to cultured human or bovine endothelial cells. At concentrations which completely blocked PMA‐stimulated adherence, PCMPS did not cause release of lactic dehydrogenase, inhibit PMA‐mediated degranulation or hydrogen peroxide production, or prevent the PMA‐induced increased surface expression of CD11b/CD 18 (Mac‐1). Coincubation with a competing reduced sulfhydryl compound protected neutrophils from inhibition of PMA‐stimulated adherence by PCMPS, whereas coincubation with an oxidized sulfhydryl compound did not. Monobromotrimethylammoniobimane, a nonpenetrating sulfhydryl reagent that is structurally unrelated to PCMPS, also inhibited stimulated neutrophil adherence to endothelium cultures. We conclude that stimulated neutrophil adherence to endothelium involves neutrophil membrane protein sulfhydryl groups. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0741-5400 1938-3673 |
DOI: | 10.1002/jlb.45.3.177 |