Inhibitory effect against polymerase and ribonuclease activities of HIV-reverse transcriptase of the aqueous leaf extract of Terminalia triflora

• Published in: Phytotherapy research Vol. 16; no. 8; pp. 778 - 780
• Main Authors: Martino, V. S., López, P., Martinez Irujo, J. J., Sanromán, M., Cuevas, M. T., Santiago, E., Lasarte, J. J., Font, M., Coussio, J. D., Monge, A.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.12.2002; Wiley
• Subjects: Anti-HIV Agents - administration & dosage; Anti-HIV Agents - pharmacology; Anti-HIV Agents - therapeutic use; antiHIV-reverse transcriptase activity; aqueous extract; Biological and medical sciences; Cell Line - drug effects; Cell Line - enzymology; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; General pharmacology; HIV Infections - drug therapy; HIV Reverse Transcriptase - antagonists & inhibitors; HIV-1 - drug effects; HIV-1 - enzymology; Humans; Inhibitory Concentration 50; Medical sciences; Nucleic Acid Synthesis Inhibitors; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Leaves; Reverse Transcriptase Inhibitors - administration & dosage; Reverse Transcriptase Inhibitors - pharmacology; Reverse Transcriptase Inhibitors - therapeutic use; Ribonucleases - drug effects; Terminalia; Terminalia triflora; Europe; Spain; Cell culture; RNA-directed DNA polymerase; HPLC chromatography; Extract; Medicinal plant; antiHIV-reverse transcriptase activity; Polyphenol; Tannin; Dicotyledones; Angiospermae; Antiviral; Enzyme; Pharmacognosy; Transferases; Retroviridae; Plant leaf; Terminalia triflora; Lentivirus; In vitro; Biological activity; Virus; Nucleotidyltransferases; Combretaceae; Plant origin; Isolation; Active ingredient; Spermatophyta; Human immunodeficiency virus; aqueous extract
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.1065

Dichloromethane, methanol and aqueous extracts from the leaves of Terminalia triflora were investigated for their inhibitory effect on polymerase and ribonuclease activities of HIV reverse transcriptase.The most potent activity was found in the aqueous extract, which inhibited both polymerase and ribonuclease activities of the enzyme with an IC50 of 1.6 μg/mL and 1.8 μg/mL respectively. The antiinfective activity of the extract was demonstrated in HLT4LacZ‐IIIB cell culture with an IC50 of 1.0 μg/mL. The extract was submitted to a purification process by extractive and chromatographic methods. The activity remained in the hydrophillic fraction. Tannins present in this active purified fraction, as determined by TLC and HPLC methods, could account for the anti HIV‐RT activity found in the aqueous extract. Copyright © 2002 John Wiley & Sons, Ltd.