Multiresidue method for the analysis of more than 140 pesticide residues in fruits and vegetables by gas chromatography coupled to triple quadrupole mass spectrometry

• Published in: Journal of mass spectrometry. Vol. 43; no. 9; pp. 1235 - 1254
• Main Authors: Fernández Moreno, José Luis, Garrido Frenich, A., Plaza Bolaños, Patricia, Martínez Vidal, José Luis
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2008; Wiley
• Subjects: Biological and medical sciences; food commodities; Food Contamination - prevention & control; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; gas chromatography; Gas Chromatography-Mass Spectrometry - methods; multiresidue analysis; pesticide; Pesticide Residues - analysis; QuEChERS extraction; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; triple quadrupole mass spectrometry (QqQ); Vegetables - chemistry; Trace analysis; Validation; Solid phase extraction; Tandem mass spectrometry; Chemical analysis; Vegetables; Fruit; Coupled method; pesticide; Pesticides; triple quadrupole mass spectrometry (QqQ); Solvent extraction; multiresidue analysis; Contamination; Quadrupole spectrometer; Chemical enrichment; QuEChERS extraction; Gas chromatography; Multicomponent analysis; Sample preparation; Residue; food commodities; Quantitative analysis
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.1400

A new multiresidue method has been developed and validated for the determination of more than 140 pesticide residues in cucumber and orange by gas chromatography coupled to triple quadrupole mass spectrometry (GC–QqQ‐MS/MS) in a single run of 25.50 min. The triple quadrupole (QqQ) analyzer simultaneously operated in the selected reaction monitoring (SRM) and selected ion monitoring (SIM) modes, acquiring two or three transitions per compound. Samples were extracted by the application of a single‐phase extraction of 10 g of sample with acetonitrile containing 1% of acetic acid, followed by a liquid–liquid partition formed by the addition of 4 g of MgSO4 and 1 g of NaOAc. A dispersive solid‐phase extraction (D‐SPE) with primary secondary amine (PSA) was applied to clean up the extracts. A final concentration step was included in order to increase sensitivity in the instrumental analysis. The method was properly validated in each matrix in a wide dynamic range (10–400 µg kg−1): this work relies on a new quantification strategy by the use of two calibration curves to increase the dynamic range, which permitted reduction of sample dilutions and increase in sample throughput. Recovery was studied at three concentration levels (11.5, 50.0, and 150.0 µg kg−1), yielding values in the range 70–110% with precision values, expressed as relative standard deviation (RSD), lower than 20 and 25% for the intraday and interday precision, respectively. Limits of quantification (LOQs) were established at 10 µg kg−1, the lowest maximum residue level (MRL) value set by the European Union in vegetables. The method was successfully applied to the analysis of pesticide residues in real samples from the southeastern Spain. Copyright © 2008 John Wiley & Sons, Ltd.