Action of pheromone biosynthesis activating neuropeptide on pheromone glands of Bombyx mori and Spodoptera litura

• Published in: Journal of Pesticide Science Vol. 17; no. 1; pp. 47 - 54
• Main Authors: Fonagy, A. (Institute of Physical and Chemical Research, Wako, Saitama (Japan)), Matsumoto, S, Uchiumi, K, Orikasa, C, Mitsui, T
• Format: Journal Article
• Language: English
• Published: Tokyo Pesticide Science Society of Japan 01.01.1992; Japan Science and Technology Agency
• Subjects: ANIMAL GLANDS; ANIMAL GROWTH PROMOTERS; Biochemistry. Physiology. Immunology; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; BOMBYX MORI; FEROMONAS; Fundamental and applied biological sciences. Psychology; GLANDE ANIMALE; GLANDULAS ANIMALES; Insecta; Invertebrates; PHEROMONE; PHEROMONES; Physiology. Development; PROMOTEUR DE CROISSANCE; PROMOTORES DEL CRECIMIENTO; SPODOPTERA LITURA; Sex pheromone; Insecta; Biochemical compound; Biosynthesis; Bombyx mori; Experimental animal; In vitro; Neuropeptide; In vivo; Pest; Phytophagous; Synthetic product; Spodoptera litura; Activator; Arthropoda; Bombycidae; Lepidoptera; Metabolic activation; Useful animal; Noctuidae; Pheromonal gland; Invertebrata; Mechanism of action; Silkworm
• ISSN: 0385-1559; 1348-589X; 1349-0923
• DOI: 10.1584/jpestics.17.47

The effect of synthetic Bom-PBAN was studied in vivo and in vitro. In B. mori, injection of 0.01ng/female induced bombykol production and maximal production was attained at 1ng/female in vivo. Time-dependence studies revealed an optimum response after incubation for 90-120min at a dose of 0.5ng/female. In vivo, sex pheromone production was induced in B. mori at 0.5ng PBAN/150μl Grace's medium and in S. litura at 2ng/50μl concentrations. Pheromone production reached maximum at 10ng/150μl in B. mori and 15ng/50μl in S. litura, respectively. In both species, optimal incubation time was 90-120min in vitro. Induction of sex pheromone production in vitro clearly indicates that the target organ of PBAN action is the pheromone gland in B. mori and S. litura. Since sex pheromone production was also induced by calcium ionophore in vitro, Ca2+ is likely to mediate the action of PBAN.