Determination of humanized anti-Tac in human serum by a sandwich enzyme linked immunosorbent assay

• Published in: Journal of immunological methods Vol. 186; no. 1; pp. 47 - 54
• Main Authors: Fayer, Bonnie E., Soni, Peter P., Binger, M.H., Mould, Diane R., Satoh, Hiroko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.10.1995; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacokinetics; Biological and medical sciences; Biotin - chemistry; Bone Marrow Transplantation - immunology; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Graft vs Host Disease - immunology; Graft-versus-host disease; Humanized anti-Tac; Humans; IL-2; Mice; Molecular immunology; Receptors, Interleukin-2 - immunology; Recombinant Fusion Proteins - pharmacokinetics; Tac; Tac receptor; Techniques; Graft-versus-host disease; IL-2; Tac receptor; Humanized anti-Tac; Tac; ELISA; Performance evaluation; Human; Antibody; Rodentia; Graft versus host reaction; Vertebrata; Mammalia; Mouse; Serum; Detection; Pharmacokinetics; ELISA assay; Chimera
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/0022-1759(95)00131-S

A ‘sandwich’ enzyme-linked immunosorbent assay has been developed for measuring humanized anti-Tac (HAT), a humanized antibody to the IL-2 receptor on activated T cells (Tac), in human serum. The working range of this assay is 25–400 ng/ml with an overall precision of 5%. In this assay, the analyte, HAT, is sandwiched between Tac which is bound to a microtiter plate and biotinylated Tac that is conjugated to peroxidase labelled streptavidin. This assay was utilized to determine the pharmacokinetic parameters of HAT in patients with graft-versus-host disease.